The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
Blog Article
To prevent the lack of stationary section, which shortens the column’s life time, it is actually sure covalently for the silica particles. Bonded stationary phases
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
Column problems: A dirty or ruined column can result in peak broadening. Contaminants can accumulate over the column after a while, hindering analyte separation. Regularly clean up the column in accordance with the maker's instructions. If cleansing doesn't assistance, take into account changing the column.
Modifying the cellular period’s composition since the separation progresses is just one Alternative to this issue. For a reversed-phase separation we use an First mobile phase which is extra polar. Since the separation progresses, we modify the composition of cellular stage to ensure that it will become fewer polar (see Figure 12.five.6
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
Fluoxetine is another name to the antidepressant drug Prozac. The determination of fluoxetine in serum is a vital Component of monitoring its therapeutic use.
混合物で構成される試料を分離する。一般にステンレス製の筒の中に、微細な真球状の多孔質シリカゲルをアルキル基等で修飾した物を充填して用いる。分取目的であれば、粉砕シリカゲルも用いられる。
, which permits us to explore a wide range of cell phases with only 7 experiments. We get started by altering the amount of acetonitrile from the mobile period to produce the absolute best separation in the desired Evaluation time.
). Because the tubing and fittings that have the mobile period have stress restrictions, a higher back tension requires a lower move level and a longer Examination time. Monolithic columns, during which the stable assist is only one, porous rod, offer column efficiencies such as a packed capillary column even though permitting for quicker stream prices. A monolithic column—which ordinarily is comparable in check here sizing to a conventional packed column, While more compact, capillary columns also are offered—is ready by forming the mono- lithic rod inside a mould and masking it with PTFE tubing or simply a polymer resin.
). Since the tubing and fittings that have the cell section have pressure limitations, a higher back stress needs a lessen flow charge and a longer Evaluation time. Monolithic columns, through which the sound aid is a single, porous rod, give column efficiencies akin to a packed capillary column even though making it possible for for faster flow costs. A monolithic column—which generally is similar in sizing to a traditional packed column, Even though smaller sized, capillary columns also are available—is prepared by forming the mono- lithic rod within a mildew and masking it with PTFE tubing or simply a polymer resin.
- 분석물의 분리여부는 고정상(컬럼)과 이동상의 조합에 의해 결정합니다.(실제 시료 측정에서는 시료 중에 분석물 이외의 오염물질에 존재하는 경우가 많아 분석자는 그 시료의 측정에 최적인 분석 조건의 검토가 필요합니다.
Inside a gas chromatograph the tension from the compressed gasoline cylinder is enough to push the mobile section from the column. Pushing a liquid mobile period through a column, however, usually takes an awesome deal additional click here energy, generating pressures in excessive of various hundred atmospheres.
The selection of detector is dependent upon the specific desires of the Assessment, thinking about elements like sensitivity, selectivity, and compatibility Using the cellular section.
Reducing the quantity of acetonitrile and growing the amount of h2o from the mobile will boost retention periods, delivering a lot more time for you to influence a separation.